The goal of our lab is to reveal the neuronal circuit mechanisms underlying sleep and wake states and to determine the neuronal underpinnings of sleep/wake disturbances in psychiatric disorders.
To accomplish this, we combine innovative techniques to record and manipulate neuronal circuits in rodents, including in vivo calcium imaging, fiber-photometry, optogenetics, chemogenetics, circuit tracing and EEG/EMG recordings with ethologically-relevant behavioral manipulations.
EEG and EMG recordings from freely-behaving animals
EEG spectrogram during a wake-to-NREM sleep transition
GCaMP6s expression in GABAergic neurons of the LH
Fiber photometry track above the VTA
GABAergic (green) neurons in the VTA are intermingled with TH+ (red) neurons
Simultaneous EEG/EMG and fiber photometry recordings during different arousal states
Circuit tracing using rAAV2-retro GFP identifies neurons projecting to sleep/wake regulatory regions
Circuit tracing using cTRIO technology identifies inputs to sleep/wake regulatory circuits
Transiently activated neurons identified using Fos CreER::Cre-dep tdTomato mice
eYFP (green) expression in TH+ neurons (red) of the VTA
All content copyright Eban-Rothschild Lab, 2017 Department of Psychology, University of Michigan, Ann Arbor