We employ a multidisciplinary approach to uncover the neuronal mechanisms controlling sleep-wake states in health and disease.
Throughout our lives, we continuously transition between wakefulness and sleep. Both states are essential for well-being, yet each comes at the expense of the other. The capacity to adaptively regulate wake and sleep periods in response to specific homeostatic and environmental conditions, such as hunger, the presence of a potential mate, or the call of an offspring, is essential for survival.
What are the neuronal mechanisms linking motivational processes with sleep-wake regulation?
What are the neuronal substrates underlying the strong association between sleep-wake disturbances and psychiatric disorders?
The goal of our lab is to uncover the neuronal underpinnings of sleep-wake states and sleep-preparatory behaviors, and to delineate the mechanisms involved in pathological alterations in these circuits. To accomplish this, we combine innovative techniques to record and manipulate neuronal circuits in rodents, including EEG/EMG recordings, in vivo calcium imaging, optogenetics, chemogenetics, and circuit tracing with ethologically-relevant behavioral manipulations.
EEG and EMG recordings from freely-behaving animals
EEG spectrogram during a wake-to-NREM sleep transition
GCaMP6s expression in GABAergic neurons of the LH
Fiber photometry track above the VTA
GABAergic (green) neurons in the VTA are intermingled with TH+ (red) neurons
Simultaneous EEG/EMG and fiber photometry recordings during different arousal states
Circuit tracing using rAAV2-retro GFP identifies neurons projecting to sleep/wake regulatory regions
Circuit tracing using cTRIO technology identifies inputs to sleep/wake regulatory circuits
Transiently activated neurons identified using Fos CreER::Cre-dep tdTomato mice
eYFP (green) expression in TH+ neurons (red) of the VTA
All content copyright Eban-Rothschild Lab, 2017 Department of Psychology, University of Michigan, Ann Arbor